Microscopy and Imaging Techniques: Fluorescence and Immunofluorescence
نویسندگان
چکیده
منابع مشابه
Spectral imaging fluorescence microscopy.
The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging ...
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We constructed an optical system and developed the software to create and analyze the fluorescence anisotropy images of a variety of objects including single molecules, quantum dots, proteins, nucleic acids and cells. The system includes an inverted microscope, three calcite prisms (CPs) inserted in the excitation and emission optical paths, and CCD camera. The system allows to record dynamic c...
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Total internal reflection fluorescence microscopy (TIRFM) is an optical technique that allows imaging of a thin layer of the sample with a thickness of about 100-200 nm. It is used in science of cell biology to study cellular processes, especially near the membranes of living cells. This method is based on the total internal reflection phenomenon, where the evanescent wave is generated in the l...
متن کاملFluorescence and fluorescence microscopy
The concept of specific cellular antigen staining by use of an immunofluorescent technique was introduced by AH COONS and co-workers about 60 years ago. Fluorescent probes are efficient tools and enable the detection of particular components in complex structures of organs including live cells. Under the condition of specific molecular interactions, fluorochrome labeled ligands allow the select...
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Optical switch probes undergo rapid and reversible transitions between two distinct states, one of which may fluoresce. This class of probe is used in various super-resolution imaging techniques and in the high-contrast imaging technique of optical lock-in detection (OLID) microscopy. Here, we introduce optimized optical switches for studies in living cells under standard conditions of cell cul...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2009
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113166